ABSTRACT
Community acquired pneumonia (CAP) is an important cause of death in children under five years old.Viruses and bacteria are common causes.At present, accurate diagnosis of the pathogen of cap in children is still a clinical challenge.Clinical diagnosis of pathogens mainly through the collection of various samples (such as nasopharyngeal swab, sputum, blood, body fluids, etc.) for pathogen detection (such as antigen, antibody, culture, polymerase chain reaction (PCR) detection). Different samples and different detection methods have their own advantages and disadvantages, especially in the diagnosis of viral pneumonia and bacterial pneumonia, which lead to great differences in treatment measures.In order to identify different pathogens early and reduce the irrational use of antibiotics, clinicians and researchers are still exploring methods regarding early accurate pathogen diagnosis.Metabonomics is a rapidly developing field, which aims to identify and quantify the concentration changes of all metabolites or metabonomics in model systems.Most disease states are related with metabolic homeostasis.Metabonomics can provide clues for the discovery of new biomarkers, and also be used for the study of infections caused by different pathogens.Non targeted metabonomics analysis provides a new diagnostic approach for the etiology of cap in children.
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Objective To study the therapeutic effect of tianluo decoction against hepatitis B virus(HBV) in vivo and vitro.Methods The inhibitive effect were observed in the duplication process of hepatitis B virus by fluorescence quantitative PCR(FQ-PCR) in vitro test and duck's hepatitis B animal model in vivo test.Results FQ-PCR in vitro test demonstrated that tianluo decoction had biological function to inhibit from duplication of hepatitis B virus,and there were significant difference in inhibit hepatitis B virus from duplicating in distilled water and tianluo decoction group(median:6.9?108/ml vs 2.6?104/ml,u=2.76,P
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Objective To investigate the enhanced effect of polysaccharide from B.jiangsiensis(PBJ) on the immune hypofunction mouse models and its dose-effect relationship.Methods The immune hypofunction mouse models were made by hypodermic injection with cyclophosphamide(Cy) in mice.PBJ's effect on hemolysin content in mouse body induced by chicken erythrocytes and the delayed-type hypersensitivity(DTH) induced by dinitrochlorobenzene in mice were observed and contrasted in the different mouse groups treated with Cy in vivo by hypodermic injection together with PBJ at doses of 400mg/kg,200mg/kg and 50mg/kg,respectively.Results The test showed that the index of hemolysin,hemolytic plaque formation and delayed hypersensitivity reaction in model group markedly decreased while the indexes of hemolysin test in the control group,high and middle dose groups increased(P
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Objective To investigate the relationship among the expressions of CD44v6,nm23 and MMP-9 and lymph node metastasis,differentiated degree as well as invasion depth in primary gastric carcinoma.Methods Seventy cases of gastric carcinoma,20 cases of chronic superficial gastritis,20 cases of large intesinal metaplasia and 20 cases of atipical hyperplasia were investigated using antibodies to CD44v6,MMP-9 and nm23 by S-P immunohistochemical technique.Results The positivity rates of CD44v6,MMP-9 and nm23 in gastric cancer were 64.3%,60.0%,57.1%,respectively.The positivity rates of CD44v6 and MMP-9 in gastric carcinoma were higher than that in the control groups.The positivity rates of nm23 in gastric carcinoma were lower than that in the control groups.The difference between the expression of CD44v6,MMP-9 and nm23 and lymph node metastasis was statistically significant.There was statistically significant difference between the expression of MMP-9,nm23 and the depth of invasion.CD44v6 expression in gastric cancer was positively correlated with MMP-9 expression(P